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AC16;人心肌细胞

Cell line name AC16 [Human hybrid cardiomyocyte]
Accession CVCL_4U18
Resource Identification Initiative To cite this cell line use: AC16 [Human hybrid cardiomyocyte] (RRID:CVCL_4U18)
Comments Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); PTA-1500.
Characteristics: Hybrid of adult ventricular cardiomiocytes with a SV40 transformed, uridine auxotroph human fibroblast cell line devoid of mitochondrial DNA (PubMed=15913645).
Characteristics: Can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes (Millipore).
Doubling time: 24.49 hours (PubMed=15913645).
Transfected with: UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from sampling site: Heart; ventricle. Cell type=Cardiomyocyte.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_4U14 (DWFb1-rho0)
Category Hybrid cell line
STR profile Source(s): Millipore
Markers:
Amelogenin X
CSF1PO 9,11,12
D3S1358 17,18
D5S818 9,11
D7S820 10,11,12
D8S1179 12,14
D13S317 12,13
D16S539 11,13
D18S51 12,17
D21S11 32.2,33.2
FGA 21,25
Penta D 2.2,9
Penta E 7,8,16
TH01 7,8,9.3
TPOX 11
vWA 16,18

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Publications

PubMed=15913645; DOI=10.1016/j.yjmcc.2005.03.003
Davidson M.M., Nesti C., Palenzuela L., Walker W.F., Hernandez E., Protas L., Hirano M., Isaac N.D.
Novel cell lines derived from adult human ventricular cardiomyocytes.
J. Mol. Cell. Cardiol. 39:133-147(2005)

Patent=US7223599
Davidson M.M.
Immortalization of human post-mitotic cells.
Patent number US7223599, 29-May-2007

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