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HCMEC/D3永生化人脑微血管内皮细胞[STR鉴定正确]

产品型号:BH-C249
产品规格:1x10^6cell/T25瓶
产品价格:¥1620

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C249

细胞英文(简称)

HCMEC/D3

细胞名称

HCMEC/D3;永生化人脑微血管内皮细胞【已通过STR鉴定】

背景资料

人脑微血管内皮细胞是人微血管内皮细胞通过含端粒酶逆转录SV40T慢病毒转染得到。

细胞来源

Millipore; SCC066

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

脑微血管

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

内皮细胞专用培养基 ; 37℃5% CO2

传代方法

首次建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=16141364; DOI=10.1096/fj.04-3458fje
Weksler B.B., Subileau E.-A., Perriere N., Charneau P., Holloway K., Leveque M., Tricoire-Leignel H., Nicotra A., Bourdoulous S., Turowski P., Male D.K., Roux F., Greenwood J., Romero I.A., Couraud P.-O.
Blood-brain barrier-specific properties of a human adult brain endothelial cell line.
FASEB J. 19:1872-1874(2005)

Patent=US8084254
Couraud P.-O., Romero I.A., Weksler B.B.
Human blood brain barrier model.
Patent number US8084254, 27-Dec-2011

PubMed=23531482; DOI=10.1186/2045-8118-10-16
Weksler B.B., Romero I.A., Couraud P.-O.
The hCMEC/D3 cell line as a model of the human blood brain barrier.
Fluids Barriers CNS 10:16.1-16.10(2013)

PubMed=26718731; DOI=10.1016/j.jprot.2015.12.011
Llombart V., Garcia-Berrocoso T., Bech-Serra J.J., Simats A., Bustamante A., Giralt D., Reverter-Branchat G., Canals F., Hernandez-Guillamon M., Montaner J.
Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC).
J. Proteomics 133:100-112(2016)

PubMed=30983357; DOI=10.1021/acs.molpharmaceut.9b00114
Masuda T., Hoshiyama T., Uemura T., Hirayama-Kurogi M., Ogata S., Furukawa A., Couraud P.-O., Furihata T., Ito S., Ohtsuki S.
Large-scale quantitative comparison of plasma transmembrane proteins between two human blood-brain barrier model cell lines, hCMEC/D3 and HBMEC/cibeta.
Mol. Pharm. 16:2162-2171(2019)

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