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DAMI人巨核细胞白血病细胞[STR鉴定正确]

产品型号:BH-C045
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C045

细胞英文(简称)

Dami

细胞名称

Dami人巨核细胞白血病细胞【已通过STR鉴定】

背景资料

收到细胞,请查看瓶子是否有破裂,培养基是否漏出,是否浑浊,如有请尽快和我们联系。如包装完好,取出25cm2培养瓶,75%酒精消毒,拆下封口膜,在显微镜下观察细胞形态是否正常,细胞的贴壁情况以及细胞密度,然后放入37℃5%CO2细胞培养箱中静置2-3小时,以稳定细胞状态。

细胞来源

ATCC CRL-9792

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

白血病

细胞形态

悬浮为主,少量贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=3196874; DOI=10.1182/blood.V72.6.1968.1968
Greenberg S.M., Rosenthal D.S., Greeley T.A., Tantravahi R., Handin R.I.
Characterization of a new megakaryocytic cell line: the Dami cell.
Blood 72:1968-1977(1988)

Patent=US5128245
Greenberg S.M., Handin R.I.
Establishment, characterization and differentiation of a new megakaryocytic cell line, the Dami cells.
Patent number US5128245, 07-Jul-1992

PubMed=9166871; DOI=10.1182/blood.V89.11.4238
Handin R.I.
DAMI cell line.
Blood 89:4238-4238(1997)

PubMed=9326259; DOI=10.1182/blood.V90.7.2850
MacLeod R.A.F., Dirks W.G., Drexler H.G.
Early contamination of the Dami cell line by HEL.
Blood 90:2850-2851(1997)

PubMed=9447816; DOI=10.1038/sj.leu.2400868
MacLeod R.A.F., Dirks W.G., Reid Y.A., Hay R.J., Drexler H.G.
Identity of original and late passage Dami megakaryocytes with HEL erythroleukemia cells shown by combined cytogenetics and DNA fingerprinting.
Leukemia 11:2032-2038(1997)

PubMed=10508494; DOI=10.1002/(SICI)1097-0215(19991112)83:4<555::aid-ijc19>3.0.CO;2-2
MacLeod R.A.F., Dirks W.G., Matsuo Y., Kaufmann M., Milch H., Drexler H.G.
Widespread intraspecies cross-contamination of human tumor cell lines arising at source.
Int. J. Cancer 83:555-563(1999)

PubMed=11021758; DOI=10.1038/sj.leu.2401891
Majka M., Rozmyslowicz T., Honczarenko M., Ratajczak J., Wasik M.A., Gaulton G.N., Ratajczak M.Z.
Biological significance of the expression of HIV-related chemokine coreceptors (CCR5 and CXCR4) and their ligands by human hematopoietic cell lines.
Leukemia 14:1821-1832(2000)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Drexler H.G., Dirks W.G., Matsuo Y., MacLeod R.A.F.
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

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