|
目录号 |
BH-C729 |
|
细胞英文(简称） |
LMH |
|
细胞名称 |
LMH鸡肝癌细胞 |
|
背景资料 |
收到细胞，请查看瓶子是否有破裂，培养基是否漏出，是否浑浊，如有请尽快和我们联系。如包装完好，取出25cm2培养瓶，75%酒精消毒,拆下封口膜,在显微镜下观察细胞形态是否正常，细胞的贴壁情况以及细胞密度,然后放入37℃，5%CO2细胞培养箱中静置2-3小时，以稳定细胞状态。 |
|
细胞来源 |
ATCC |
|
代次 |
P3 |
|
规格 |
冻存管/培养瓶 |
|
细胞数 |
1x10^6 cells |
|
生物安全级别 |
1 |
|
组织来源 |
肝 |
|
细胞形态 |
贴壁；上皮细胞样 |
|
细胞活力 |
95％（Viability by Trypan Blue Exclusion） |
|
细胞检测 |
细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌 |
|
培养条件 |
DMEM/F12+10% FBS（货号：C2056-1A）+1%P/S；37℃，5% CO2 |
|
传代方法 |
建议1:2-1:3 两天换液一次 |
|
冻存条件 |
PubMed=3607775
Kawaguchi T., Nomura K., Hirayama Y., Kitagawa T.
Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH.
Cancer Res. 47:4460-4464(1987)
PubMed=2330000; DOI=10.1210/mend-4-2-201
Binder R., MacDonald C.C., Burch J.B.E., Lazier C.B., Williams D.L.
Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line.
Mol. Endocrinol. 4:201-208(1990)
PubMed=2352324; DOI=10.1128/JVI.64.7.3249-3258.1990
Condreay L.D., Aldrich C.E., Coates L., Mason W.S., Wu T.-T.
Efficient duck hepatitis B virus production by an avian liver tumor cell line.
J. Virol. 64:3249-3258(1990)
PubMed=10564780; DOI=10.1016/s0304-4165(99)00159-2
Kolluri S., Elbirt K.K., Bonkovsky H.L.
Heme biosynthesis in a chicken hepatoma cell line (LMH): comparison with primary chick embryo liver cells (CELC).
Biochim. Biophys. Acta 1472:658-667(1999)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)
