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B95-8绒猴EB病毒转化的白细胞

产品型号:BH-C728
产品规格:1x10^6cell / T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C728

细胞英文(简称)

B95-8

细胞名称

B95-8绒猴EB病毒转化的白细胞

背景资料

  收到细胞,请查看瓶子是否有破裂,培养基是否漏出,是否浑浊,如有请尽快和我们联系。如包装完好,取出25cm2培养瓶,75%酒精消毒,拆下封口膜,在显微镜下观察细胞形态是否正常,细胞的贴壁情况以及细胞密度,然后放入375%CO2细胞培养箱中静置2-3小时,以稳定细胞状态。

细胞来源

DSMZ

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

2

组织来源

外周血

细胞形态

悬浮;淋巴母细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-164010% FBS1% P/S375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

相关试剂

参考文献

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Miller G., Shope T., Lisco H., Stitt D., Lipman M.
Epstein-Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes.
Proc. Natl. Acad. Sci. U.S.A. 69:383-387(1972)

PubMed=4346033; DOI=10.1073/pnas.70.1.190
Miller G., Lipman M.
Release of infectious Epstein-Barr virus by transformed marmoset leukocytes.
Proc. Natl. Acad. Sci. U.S.A. 70:190-194(1973)

PubMed=4357683; DOI=10.1084/jem.138.6.1398
Miller G., Lipman M.
Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus.
J. Exp. Med. 138:1398-1412(1973)

PubMed=6265077
Pizzo P.A., Chattopadhyay S.K., Magrath I.T., Del Giacco E., Sherrick D., Gray T.
Examination of Epstein-Barr virus and C-type proviral sequences in American and African lymphomas and derivative cell lines.
Cancer Res. 41:3165-3171(1981)

PubMed=6294325; DOI=10.1128/JVI.44.3.1088-1091.1982
Skare J., Edson C., Farley J., Strominger J.L.
The B95-8 isolate of Epstein-Barr virus arose from an isolate with a standard genome.
J. Virol. 44:1088-1091(1982)

PubMed=3017841; DOI=10.1007/BF00279094
Neitzel H.
A routine method for the establishment of permanent growing lymphoblastoid cell lines.
Hum. Genet. 73:320-326(1986)

PubMed=2824833; DOI=10.1128/JVI.61.12.4033-4037.1987
Shaw J.E., Petit R.G., Leung K.
Growth of B95-8 cells and expression of Epstein-Barr virus lytic phase in serum-free medium.
J. Virol. 61:4033-4037(1987)

PubMed=3145248
Callard R.E., Lau Y.L., Shields J.G., Smith S.H., Cairns J., Flores-Romo L., Gordon J.
The marmoset B-lymphoblastoid cell line (B95-8) produces and responds to B-cell growth and differentiation factors: role of shed CD23 (sCD23).
Immunology 65:379-384(1988)

PubMed=2153236; DOI=10.1128/JVI.64.2.700-705.1990
Kobune F., Sakata H., Sugiura A.
Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus.
J. Virol. 64:700-705(1990)

CLPUB00447
Mulivor R.A., Suchy S.F.
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda (1992)

PubMed=20454443; DOI=10.1155/2010/904767
Uphoff C.C., Denkmann S.A., Steube K.G., Drexler H.G.
Detection of EBV, HBV, HCV, HIV-1, HTLV-I and -II, and SMRV in human and other primate cell lines.
J. Biomed. Biotechnol. 2010:904767.1-904767.23(2010)

PubMed=24380390; DOI=10.1186/gb-2013-14-12-r148
Schwalie P.C., Ward M.C., Cain C.E., Faure A.J., Gilad Y., Odom D.T., Flicek P.
Co-binding by YY1 identifies the transcriptionally active, highly conserved set of CTCF-bound regions in primate genomes.
Genome Biol. 14:R148.1-R148.15(2013)

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