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CHO-K1中国仓鼠卵巢细胞

产品型号:BH-C702
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C702

细胞英文(简称)

CHO-K1

细胞名称

CHO-K1;中国仓鼠卵巢细胞

背景资料

1957年,PuckTT从成年中国仓鼠卵巢的活检组织建立了CHO细胞;广泛用于生物制品的表达。

细胞来源

ATCC CRL-9618

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

卵巢

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

F12K +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=10320750; DOI=10.1016/S0027-5107(99)00077-9
Hu T., Miller C.M., Ridder G.M., Aardema M.J.
Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing.
Mutat. Res. 426:51-62(1999)

PubMed=21804562; DOI=10.1038/nbt.1932
Xu X., Nagarajan H., Lewis N.E., Pan S.-K., Cai Z.-M., Liu X., Chen W.-B., Xie M., Wang W.-L., Hammond S., Andersen M.R., Neff N., Passarelli B., Koh W., Fan H.C., Wang J.-B., Gui Y.-T., Lee K.H., Betenbaugh M.J., Quake S.R., Famili I., Palsson B.O., Wang J.
The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line.
Nat. Biotechnol. 29:735-741(2011)

PubMed=21945585; DOI=10.1016/j.jbiotec.2011.09.014
Becker J., Hackl M., Rupp O., Jakobi T., Schneider J., Szczepanowski R., Bekel T., Borth N., Goesmann A., Grillari J., Kaltschmidt C., Noll T., Puhler A., Tauch A., Brinkrolf K.
Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing.
J. Biotechnol. 156:227-235(2011)

DOI=10.3390/pr1030296
Wurm F.M.
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

PubMed=23873082; DOI=10.1038/nbt.2624
Lewis N.E., Liu X., Li Y.-X., Nagarajan H., Yerganian G., O'Brien E., Bordbar A., Roth A.M., Rosenbloom J., Bian C., Xie M., Chen W.-B., Li N., Baycin-Hizal D., Latif H., Forster J., Betenbaugh M.J., Famili I., Xu X., Wang J., Palsson B.O.
Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
Nat. Biotechnol. 31:759-765(2013)

PubMed=26993211; DOI=10.1016/j.jbiotec.2016.03.022
Klanert G., Jadhav V., Shanmukam V., Diendorfer A.B., Karbiener M., Scheideler M., Bort J.H., Grillari J., Hackl M., Borth N.
A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.
J. Biotechnol. 235:150-161(2016)

DOI=10.3390/pr5020020
Wurm F.M., Wurm M.J.
Cloning of CHO cells, productivity and genetic stability -- a discussion.
Processes 5:20.1-20.13(2017)

PubMed=28544881; DOI=10.1016/j.cels.2017.04.009
Yusufi F.N.K., Lakshmanan M., Ho Y.-S., Loo B.L.-W., Ariyaratne P., Yang Y., Ng S.-K., Tan T.R.-M., Yeo H.C., Lim H.L., Ng S.-W., Hiu A.-P., Chow C.-P., Wan C., Chen S., Teo G., Song G., Chin J.X., Ruan X., Sung K.W.-K., Hu W.-S., Yap M.G.-S., Bardor M., Nagarajan N., Lee D.-Y.
Mammalian systems biotechnology reveals global cellular adaptations in a recombinant CHO cell line.
Cell Syst. 4:530-542.e6(2017)

PubMed=32619503; DOI=10.1016/j.ymben.2020.06.002
Szeliova D., Ruckerbauer D.E., Galleguillos S.N., Petersen L.B., Natter K., Hanscho M., Troyer C., Causon T., Schoeny H., Christensen H.B., Lee D.-Y., Lewis N.E., Koellensperger G., Hann S., Nielsen L.K., Borth N., Zanghellini J.
What CHO is made of: variations in the biomass composition of Chinese hamster ovary cell lines.
Metab. Eng. 61:288-300(2020)

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