商品详情

目录号	BH-C705
细胞英文(简称）	CHL
细胞名称	CHL；仓鼠肺细胞
背景资料	收到细胞请检查包装是否有破损，如有异常请尽快与我们联系!
细胞来源	ECACC
代次	P3
规格	冻存管/培养基
细胞数	1x10^6 cells
生物安全级别	1
组织来源	肺
细胞形态	贴壁
细胞活力	95％（Viability by Trypan Blue Exclusion）
细胞检测	细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
培养条件	DMEM(高糖) +10% FBS（货号：C2056-1A）+1%P/S；37℃，5% CO2
传代方法	建议1:2-1:3 两天换液一次
冻存条件	无血清细胞冻存液（货号：S002）

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参考文献

PubMed=5452405; DOI=10.20772/cancersci1959.61.2_161
Koyama H., Utakoji T., Ono T.
A new cell line derived from newborn Chinese hamster lung tissue.
Gann 61:161-167(1970)

PubMed=1170023; DOI=10.1016/0092-8674(75)90021-5
Miyashita K., Kakunaga T.
Isolation of heat- and cold-sensitive mutants of Chinese hamster lung cells affected in their ability to express the transformed state.
Cell 5:131-138(1975)

PubMed=688393; DOI=10.1016/0092-8674(78)90354-9
Gupta R.S., Chan D.Y.H., Siminovitch L.
Evidence for functional hemizygosity at the Emtr locus in CHO cells through segregation analysis.
Cell 14:1007-1013(1978)

PubMed=3558490; DOI=10.1002/jcp.1041300303
Gupta R.S., Dudani A.K.
Species-specific differences in the toxicity of rhodamine 123 towards cultured mammalian cells.
J. Cell. Physiol. 130:321-327(1987)

PubMed=1370240; DOI=10.1016/0027-5107(92)90036-2
Sawada M., Kitamura R., Kamataki T.
Stable expression of monkey cytochrome P-450IA1 cDNA in Chinese hamster CHL cells and its application for detection of mutagenicity of aflatoxin B1.
Mutat. Res. 265:23-29(1992)

PubMed=7512690; DOI=10.1016/0165-7992(94)90033-7
Ono T., Norimatsu M., Yoshimura H.
Induction of chromosome aberrations by pyrimethamine in cultured Chinese hamster (CHL) cells.
Mutat. Res. 323:197-201(1994)

晓洁：

CHL中国仓鼠肺细胞

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