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BV2小鼠小胶质细胞

产品型号:BH-C414
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C414

细胞英文(简称)

BV2BV-2

细胞名称

BV2BV-2小鼠小胶质细胞

背景资料

BV-2E.Blasi年建立于1990年。该细胞由小鼠小神经胶质细胞经逆转录病毒介导转染v-raf/v-myc获永生化。该细胞系保留有小神经胶质细胞多种形态,表征,和功能特征。免疫组化结果显示细胞为MAC1,MAC2阳性,而MAC3,胶质细胞原纤维酸性蛋白,半乳糖脑苷脂为阴性

细胞来源

BCRJ

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

小胶质

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM (高糖)+10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=2110186; DOI=10.1016/0165-5728(90)90073-V
Blasi E., Barluzzi R., Bocchini V., Mazzolla R., Bistoni F.
Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.
J. Neuroimmunol. 27:229-237(1990)

PubMed=19565166; DOI=10.14573/altex.2009.2.83
Henn A., Lund S., Hedtjarn M., Schrattenholz A., Porzgen P., Leist M.
The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.
ALTEX 26:83-94(2009)

PubMed=21961028; DOI=10.2174/1877381801002010148
Crain J.M., Watters J.J.
Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.
Open Drug Discov. J. 2:148-167(2010)

PubMed=21214938; DOI=10.1186/1471-2164-12-14
Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14.1-14.14(2011)

PubMed=22651808; DOI=10.1186/1742-2094-9-115
Stansley B., Post J., Hensley K.
A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.
J. Neuroinflamm. 9:115.1-115.8(2012)

PubMed=26227174; DOI=10.1002/pmic.201400623
Woo J.-M., Han D.-H., Park J.-H., Kim S.-J., Kim Y.-S.
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)

DOI=10.1007/s13273-019-00057-6
Mandal C., Yoon T., Park J.Y., Jung K.H., Chai Y.G.
Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells.
Mol. Cell. Toxicol. 16:51-61(2020)

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