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M-NFS-60 [NFS-60]小鼠髓性白血病淋巴细胞/小鼠白血病G-CSF依赖性细胞

产品型号:BH-C449
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C449

细胞英文(简称)

M-NFS-60

细胞名称

M-NFS-60小鼠髓性白血病淋巴细胞

背景资料

收到细胞请检查包装是否有破损,如有异常请尽快与我们联系!

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

淋巴

细胞形态

悬浮;淋巴母细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640+10% FBS(货号:C2056-1A+15ng/ml G-CSF+1% P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=3014527; DOI=10.1073/pnas.83.14.5010
Weinstein Y., Ihle J.N., Lavu S., Reddy E.P.
Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line.
Proc. Natl. Acad. Sci. U.S.A. 83:5010-5014(1986)

PubMed=2497280
Gascan H., Moreau J.-F., Jacques Y., Soulillou J.-P.
Response of murine IL3-sensitive cell lines to cytokines of human and murine origin.
Lymphokine Res. 8:79-84(1989)

PubMed=2656885; DOI=10.1002/stem.5530070202
Ihle J.N., Askew D.
Origins and properties of hematopoietic growth factor-dependent cell lines.
Int. J. Cell Cloning 7:68-91(1989)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

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