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MRC-5人胚肺成纤维细胞[STR鉴定正确]

产品型号:BH-C216
产品规格:1x10^6cell/T25瓶
产品价格:¥1620

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C216

细胞英文(简称)

MRC-5

细胞名称

MRC-5人胚肺成纤维细胞【已通过STR鉴定】

背景资料

MRC-5细胞系来自14周龄男性胎儿的正常肺组织,该细胞老化前能传代4246次群体倍增。 它是正常二倍体细胞系,46XY核型。 模式染色体数为46,概率为70%

细胞来源

ATCC CCL-171

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁;成纤维细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

MEM+10% FBS(货号:C2056-1A+1% P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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Characteristics of a human diploid cell designated MRC-5.
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The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines.
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PubMed=1031681
Jacobs J.P.
Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains.
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Friedman H.M., Koropchak C.
Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.
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Cultured cell lines for research on pulmonary physiology available through the American Type Culture Collection.
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4F2 monoclonal antibody recognizes a surface antigen on spread human fibroblasts of embryonic but not of adult origin.
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Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.
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PubMed=1965304; DOI=10.1002/elps.1150111203
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The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells.
Electrophoresis 11:1072-1113(1990)

PubMed=10752125
Polianskaia G.G., Efremova T.N., Sakuta G.A.
The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability.
Tsitologiia 42:190-195(2000)

PubMed=17395773; DOI=10.1634/stemcells.2006-0504
Sudo K., Kanno M., Miharada K., Ogawa S., Hiroyama T., Saijo K., Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells 25:1610-1617(2007)

PubMed=19657000
Rubporn A., Srisomsap C., Subhasitanont P., Chokchaichamnankit D., Chiablaem K., Svasti J., Sangvanich P.
Comparative proteomic analysis of lung cancer cell line and lung fibroblast cell line.
Cancer Genomics Proteomics 6:229-237(2009)

PubMed=21637780; DOI=10.1371/journal.pgen.1002085
Nishino K., Toyoda M., Yamazaki-Inoue M., Fukawatase Y., Chikazawa E., Sakaguchi H., Akutsu H., Umezawa A.
DNA methylation dynamics in human induced pluripotent stem cells over time.
PLoS Genet. 7:E1002085-E1002085(2011)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=26477663; DOI=10.1038/srep14988
Ojima T., Shibata E., Saito S., Toyoda M., Nakajima H., Yamazaki-Inoue M., Miyagawa Y., Kiyokawa N., Fujimoto J.I., Sato T., Umezawa A.
Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.
Sci. Rep. 5:14988-14988(2015)

CLPUB00391
Wadman M.
The vaccine race: science, politics, and the human costs of defeating disease.
(In) ISBN 9780525427537; pp.1-436; Viking; New York (2016)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
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Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

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