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AU565人乳腺癌细胞[STR鉴定正确]

产品型号:BH-C230
产品规格:1x10^6cell/T25瓶
产品价格:¥1620

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商品详情

目录号

BH-C230

细胞英文(简称)

AU565

细胞名称

AU565人乳腺癌细胞【已通过STR鉴定】

背景资料

AU565 细胞系源自加利福尼亚州奥克兰海军生物科学实验室的乳腺癌患者胸腔积液。该细胞系是从与 SK-BR-3 ( ATCC HTB-30 ) 相同的患者建立的。该患者是一名白人女性,43 岁,A+ 型血,曾接受放射、类固醇、环磷酰胺和 5-氟尿嘧啶治疗。AU565细胞系扩增并过表达HER-2/neu癌基因;它表达 HER-3、HER-4 和 p53 癌基因。用霉酚酸 (MPA)、佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA)、视黄酸 (RA) 或针对 HER-2/neu 蛋白胞外结构域的 TA-1 单克隆抗体处理细胞可诱导细胞分化。用 Neu 分化因子 (NDF) 处理 AU565 细胞也会诱导成熟表型,其特征在于细胞的形态外观。未经处理的 AU565 细胞具有致密的细胞核和薄的细胞质,而处理的细胞则表现出与分化表型相关的形态,例如被相当大的细胞质包围的扁平大细胞核。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

乳腺

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

首次建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

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