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RWPE-1人前列腺正常细胞[STR鉴定正确]

产品型号:BH-C288
产品规格:1x10^6cell/T25瓶
产品价格:¥1800

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商品详情

目录号

BH-C288

细胞英文(简称)

RWPE-1

细胞名称

RWPE-1人前列腺上皮细胞

背景资料

该细胞来源于54岁白人男性。源于正常成人前列腺周边组织的上皮细胞经过转染HPV-18后建系得到该细胞株。在三维基质胶培养时,在雄激素作用下,RWPE-1细胞形成腺胞并向培养基中分泌PSA(kallikrein 3, KLK3)。来源于RWPE-1的细胞再用Kirstin鼠类肿瘤病毒转染Ki-ras基因,建立了能成瘤的RWPE-2细胞株和 RWPE2-W99细胞株。同时,用N-甲醇-N-硝基脲处理RWPE-1,建立了一系列模拟前列腺癌进程中不同时期的成瘤细胞株, 分别是 WPE1-NA22, WPE1-NB14, WPE1-NB11 和 WPE1-NB26 细胞株。 据提供者报道,RWPE-1 细胞株经过检测,乙肝、丙肝、人免疫缺陷病毒都呈阴性。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

2

组织来源

前列腺

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

K-SFM培养基 GIBCO货号(10744019)37℃,5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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