在线客服

晓洁:

198-8659-7060

HET-1A人食管上皮细胞[STR鉴定正确]

产品型号:BH-C289
产品规格:1x10^6cell/T25瓶
产品价格:¥2450

返回列表

商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C289

细胞英文(简称)

Het-1A

细胞名称

Het-1A人食管上皮细胞【已通过STR鉴定】

背景资料

HET-1A 细胞系于 1986 年通过用由 RSV-LTR 启动子和编码猿猴病毒 40 大 T 抗原的序列组成的质粒 pRSV-T 转染从人食管尸检组织中衍生而来。生长因子研究表明,HET-1A 受钙刺激,受胎牛血清、转化生长因子-β1 和转化生长因子-β2 抑制。伏马菌素 B1 可抑制 HET-1A 细胞的生长并增加细胞凋亡。合成类视黄醇 CD437(6-[3-(1-金刚烷基)-4-羟基苯基]-2-萘甲酸 (AHPN/CD437)0)通过 caspase-3 依赖途径诱导食管鳞状 HET-1A 细胞凋亡。细胞核型位亚二倍体(34-40 条染色体),可用于研究假定的食道致癌物的作用。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

食管

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

BEBM kit:CC-317037℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

CLPUB00265
Stoner G.D., Kaighn M.E., Resau J.H., Naito Z., Bowman D., Harris C.C.
Immortalized human esophageal epithelial cell lines for studies of carcinogen-induced cell transformation.
In Vitro Cell. Dev. Biol. 26:23A-23A(1990)

DOI=10.1007/978-1-4612-0411-4_30
Stoner G.D., Light B.A., Harris C.C.
Immortalization and oncogene transformation of human esophageal epithelial cells.
(In) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.297-305; Humana Press; New York (1990)

Patent=WO1991005062A1
Stoner G.D., Reddel R.R., Harris C.C.
Human esophageal epithelial cell lines.
Patent number WO1991005062A1, 18-Apr-1991

PubMed=1703038
Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C.
Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells.
Cancer Res. 51:365-371(1991)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

PubMed=7972006; DOI=10.1073/pnas.91.23.11045
Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B., Serrano M., Beach D.H., Harris C.C.
Mutations and altered expression of p16INK4 in human cancer.
Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

PubMed=10078940; DOI=10.1002/(SICI)1098-2744(199902)24:2<128::aid-mc7>3.0.co;2-f
Compton K.R., Orringer M.B., Beer D.G.
Induction of glutathione s-transferase-pi in Barrett's metaplasia and Barrett's adenocarcinoma cell lines.
Mol. Carcinog. 24:128-136(1999)

PubMed=20139130; DOI=10.1093/carcin/bgq036
Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.
An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.
Carcinogenesis 31:936-945(2010)

PubMed=20843300; DOI=10.1042/BC20100071
Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P.
A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture.
Biol. Cell 102:635-644(2010)

PubMed=21127259; DOI=10.1152/ajpgi.00461.2010
Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K.
Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.
Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)

PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002
Akagi I., Ishibashi O.
RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.
Genomics Data 12:4-6(2017)

联系我们
  • 19886597060