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NCI-H522人非小细胞肺癌腺癌细胞[STR鉴定正确]

产品型号:BH-C291
产品规格:1x10^6cell/T25瓶
产品价格:¥1620

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商品详情

目录号

BH-C291

细胞英文(简称)

NCI-H522

细胞名称

NCI-H522;人非小细胞肺癌腺癌细胞【已通过STR鉴定】

背景资料

该细胞系是由A.F. Gazdar、H.K. Oie、J.D. Minna及其同事从一名58岁白色男性吸烟肺癌患者治疗前获得的。 这是一种亚三倍体人类细胞系,其模态染色体数出现在 68% 的计数细胞中。多倍体细胞发生率为 3.0%;所有细胞共有 11 条标记染色体,其中两条是配对的。标记包括:der(6)(q13)、t(7p17q)、del(10)(p11.23)、配对 der(7)t(7;?17)(p22;?q21.1) 和 der( 16)t(16;?)(q24;?) 和其他六个;正常 Y、N13、N15 和 N16 不存在;X染色体大多是成对的。这些细胞携带 TP53 基因密码子 191 的突变。细胞不能合成肽神经调节蛋白 B (NMB) 或胃泌素释放肽 (GRP)。细胞对角蛋白和波形蛋白染色呈阳性,但对神经丝三联体蛋白呈阴性。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640+10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

首次建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

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Cancer Cell 40:835-849.e8(2022)

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