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293(HEK-293)人胚肾细胞[STR鉴定正确]

产品型号:BH-C015
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C015

细胞英文(简称)

HEK-293

细胞名称

293(HEK-293) 人胚肾细胞【已通过STR鉴定】

背景资料

293细胞是剪切过的人腺病毒5(Ad5)转染的人胚肾细胞形成的永生化细胞。 此细胞包含并表达转染的Ad5基因。早期报道中指出该细胞基因组中含有腺病毒5Ad5)基因组的左侧端和右侧端的DNA,但是现在明确了只存在其左侧端的DNA。经过对Ad5的插入点的克隆测序发现,Ad514344位线性核苷酸整合入细胞染色19q13.2。该细胞为人类腺病毒载体扩增的宿主。可表达异常的玻连蛋白的细胞表面受体,由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。 需在生物安全2级实验室操作。

细胞来源

ATCC CRL1573

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

肾;以腺病毒5 DNA 进行转化

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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