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ARPE-19人视网膜色素上皮细胞[STR鉴定正确]

产品型号:BH-C030
产品规格:1×10^6cell/T25瓶
产品价格:¥2100

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C030

细胞英文(简称)

ARPE-19

细胞名称

ARPE-19人视网膜上皮细胞【已通过STR鉴定】

背景资料

细胞来源

ATCC CRL-2302

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

视网膜

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM/F12+10% FBS(货号:C2056-1A+1% P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=8698076; DOI=10.1006/exer.1996.0020
Dunn K.C., Aotaki-Keen A.E., Putkey F.R., Hjelmeland L.M.
ARPE-19, a human retinal pigment epithelial cell line with differentiated properties.
Exp. Eye Res. 62:155-169(1996)

PubMed=11504951; DOI=10.1073/pnas.171266298
Lund R.D., Adamson P., Sauve Y., Keegan D.J., Girman S.V., Wang S., Winton H.L., Kanuga N., Kwan A.S.L., Beauchene L., Zerbib A., Hetherington L., Couraud P.-O., Coffey P., Greenwood J.
Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Proc. Natl. Acad. Sci. U.S.A. 98:9942-9947(2001)

PubMed=14551534
Rogojina A.T., Orr W.E., Song B.K., Geisert E.E. Jr.
Comparing the use of Affymetrix to spotted oligonucleotide microarrays using two retinal pigment epithelium cell lines.
Mol. Vis. 9:482-496(2003)

PubMed=16262907; DOI=10.1186/1471-2415-5-25
Sharma R.K., Orr W.E., Schmitt A.D., Johnson D.A.
A functional profile of gene expression in ARPE-19 cells.
BMC Ophthalmol. 5:25.1-25.10(2005)

PubMed=21697133; DOI=10.1167/iovs.11-7479
Oshikawa M., Tsutsui C., Ikegami T., Fuchida Y., Matsubara M., Toyama S., Usami R., Ohtoko K., Kato S.
Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011)

DOI=10.5897/IJBMBR2013.0154
Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=24251032; DOI=10.1155/2013/216359
Pasovic L., Utheim T.P., Maria R., Lyberg T., Messelt E.B., Aabel P., Chen D.F., Chen X., Eidet J.R.
Optimization of storage temperature for cultured ARPE-19 cells.
J. Ophthalmol. 2013:216359-216359(2013)

PubMed=25177495; DOI=10.1155/2014/801787
Kuznetsova A.V., Kurinov A.M., Aleksandrova M.A.
Cell models to study regulation of cell transformation in pathologies of retinal pigment epithelium.
J. Ophthalmol. 2014:801787-801787(2014)

PubMed=27499609
Wang Y., Sang A.-M., Zhu M.-H., Zhang G.-W., Guan H.-J., Ji M., Chen H.
Tissue factor induces VEGF expression via activation of the Wnt/beta-catenin signaling pathway in ARPE-19 cells.
Mol. Vis. 22:886-897(2016)

PubMed=28978645; DOI=10.1074/jbc.M117.812677
Chiang C.-K., Tworak A., Kevany B.M., Xu B., Mayne J., Ning Z., Figeys D., Palczewski K.
Quantitative phosphoproteomics reveals involvement of multiple signaling pathways in early phagocytosis by the retinal pigmented epithelium.
J. Biol. Chem. 292:19826-19839(2017)

PubMed=29476476; DOI=10.1007/978-1-4939-7680-5_17
Fasler-Kan E., Aliu N., Wunderlich K., Ketterer S., Ruggiero S., Berger S., Meyer P.
The retinal pigment epithelial cell line (ARPE-19) displays mosaic structural chromosomal aberrations.
Methods Mol. Biol. 1745:305-314(2018)

PubMed=31254428; DOI=10.1111/ceo.13578
Churm R., Dunseath G.J., Prior S.L., Thomas R.L., Banerjee S., Owens D.R.
Development and characterization of an in vitro system of the human retina using cultured cell lines.
Clin. Exp. Ophthalmol. 47:1055-1062(2019)

PubMed=31412689; DOI=10.3390/pharmaceutics11080412
Hellinen L., Pirskanen L., Tengvall-Unadike U., Urtti A., Reinisalo M.
Retinal pigment epithelial cell line with fast differentiation and improved barrier properties.
Pharmaceutics 11:412.1-412.12(2019)

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