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L-02(HL-7702)人正常肝细胞[STR鉴定正确]

产品型号:BH-C073
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C073

细胞英文(简称)

HL-7702(L-02)

细胞名称

HL-7702(L-02)人肝细胞

背景资料

L-02细胞建系鉴定于1980年。该细胞是一株正常肝细胞系,具有典型的肝细胞形态学特征,可用于多种实验研究。经STR验证公示表明被HELA细胞污染或是其衍生物。

细胞来源

中科院

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=23498809; DOI=10.1016/j.transproceed.2012.09.121
Hu X., Yang T., Li C., Zhang L., Li M., Huang W., Zhou P.
Human fetal hepatocyte line, L-02, exhibits good liver function in vitro and in an acute liver failure model.
Transplant. Proc. 45:695-700(2013)

PubMed=23505090; DOI=10.1002/hep.26402
Wang K., Lim H.Y., Shi S., Lee J., Deng S., Xie T., Zhu Z., Wang Y., Pocalyko D., Yang W.J., Rejto P.A., Mao M., Park C.-K., Xu J.
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=26116706; DOI=10.1096/fj.14-266718
Ye F., Chen C.-G., Qin J., Liu J., Zheng C.-Y.
Genetic profiling reveals an alarming rate of cross-contamination among human cell lines used in China.
FASEB J. 29:4268-4272(2015)

PubMed=26143199; DOI=10.1016/j.jhazmat.2015.06.031
Cui R., Zhang H.-X., Guo X.-J., Cui Q.-Q., Wang J.-S., Dai J.-Y.
Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.
J. Hazard. Mater. 299:361-370(2015)

PubMed=27027780; DOI=10.1007/s10565-016-9316-2
Wu Y., Geng X.-C., Wang J.-F., Miao Y.-F., Lu Y.-L., Li B.
The HepaRG cell line, a superior in vitro model to L-02, HepG2 and hiHeps cell lines for assessing drug-induced liver injury.
Cell Biol. Toxicol. 32:37-59(2016)


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