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MT-4人急性淋巴母细胞白血病细胞[STR鉴定正确]

产品型号:BH-C088
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C088

细胞英文(简称)

MT-4

细胞名称

MT-4;人急性淋巴母细胞白血病细胞【已通过STR鉴定】

背景资料

HTLV-I转化细胞白细胞,HTLV-I抗原阳性。

细胞来源

JCRB

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

淋巴

细胞形态

悬浮

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S+2-巯基乙醇0.05mM 37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

CLPUB00542
Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T.
Type C virus-producing cell lines derived from adult T cell leukemia.
Gann Monogr. Cancer Res. 28:219-228(1982)

PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8
Harada S., Koyanagi Y., Yamamoto N.
Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events.
Virology 146:272-281(1985)

PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t
Orita S., Saiga A., Takagi S., Tanaka T., Okumura K., Aono Y., Hinuma Y., Igarashi H.
A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein.
FEBS Lett. 295:127-134(1991)

PubMed=1364232
Gyuris A., Vajda G., Foldes I.
Establishment of an MT4 cell line persistently producing infective HIV-1 particles.
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PubMed=8927062
Susloparov M.A., Krendel'shchikov A.V., Bakhtina M.M., Bedristov A.I., Babkin I.V., Gutorov V.V., Eroshkin A.M., Mamaeva O.A.
Cloning of proviral DNA sequences of the human T-cell leukemia virus type 1 (HTLV-1) from the genome of cultured MT-4 cells.
Mol. Genet. Mikrobiol. Virusol. 2:32-39(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x
Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N., Liu C., Kondo E., Minowada J., Akagi T.
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997
Jeang K.-T., Derse D.D., Matocha M., Sharma O.
Expression status of Tax protein in human T-cell leukemia virus type 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH AIDS Research and Reference Reagent Program.
J. Virol. 71:6277-6278(1997)

PubMed=29892436; DOI=10.1098/rsos.172472
Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=31554688; DOI=10.1128/JVI.01390-19
Fernandez M.V., Delviks-Frankenberry K.A., Scheiblin D.A., Happel C., Pathak V.K., Freed E.O.
Authentication analysis of MT-4 cells distributed by the NIH AIDS Reagent Program.
J. Virol. 93:e01390.19-e01390.19(2019)

PubMed=32938764; DOI=10.1128/JVI.01334-20
Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.
Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.
J. Virol. 94:e01334.20-e01334.20(2020)

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