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NCI-H292人肺癌细胞(淋巴结转移)[STR鉴定正确]

产品型号:BH-C097
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C097

细胞英文(简称)

NCI-H292

细胞名称

NCI-H292人肺癌细胞【已通过STR鉴定】

背景资料

该细胞株源自肺支气管黏液上皮样癌的淋巴结转移灶;先用成份限定的培养基分离得到,然后换成含血清的培养基培养。培养条件下该细胞保留了黏液上皮的特性,可从其超微结构的表现和多种鳞状上皮分化标记的表达而判断。该细胞支持HBV的生长,左旋多巴脱羧酶阴性。该细胞可以作为筛选模型,将人类亚基因组片段转染入细胞来研究HBV及其自身基因在病毒性肝炎和肝癌发生中的作用。该细胞角蛋白、波形蛋白、黏液洋红染色阳线,但神经丝三联蛋白阴性。

细胞来源

ATCC CRL-1848

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

肺粘膜上皮细胞癌;肺

细胞形态

贴壁,上皮样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

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