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NOZ人胆囊癌细胞[STR鉴定正确]

产品型号:BH-C102
产品规格:1×10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C102

细胞英文(简称)

NOZ

细胞名称

NOZ;人胆囊癌细胞【已通过STR鉴定】

背景资料

患者有癌性腹膜炎。细胞为中等分化的管状胆囊癌。会分泌AFPCEA。倍增时间48小时,板植率14-19%。细胞可在裸鼠中成瘤,形态与原发肿瘤相似。

细胞来源

JCRB1033

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

胆囊

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

CLPUB00469
Homma S.
Studies on the establishment and some biological characteristics of cultured human liver cancer cell lines. Their growth, functional and morphological characteristics and temperature sensitivities.
Jikeikai Med. J. 32:289-315(1985)

PubMed=3154020
Homma S., Hasumura S., Nagamori S., Kameda H.
Establishment and characterization of a human gall bladder carcinoma cell line NOZ.
Hum. Cell 1:95-97(1988)

PubMed=2543327
Hasumura S., Nagamori S., Fujise K., Homma S., Sujino H., Matsuura T., Shimizu K., Niiya M., Kameda H.
Combination therapy of hyperthermia and other methods in liver and bile tract cancers -- evaluation of these methods using cancer cell lines in vitro.
Gan To Kagaku Ryoho 16:1905-1912(1989)

DOI=10.1007/BF02348185
Kawada M., Aizaki H., Fukaya K.-I., Niiya M., Matsuura T., Sujino H., Hasumura S., Nagamori S., Toda G.
Morphological changes in human gall bladder carcinoma cell line-NOZ due to epirubicin and doxorubicin.
Med. Electron Microsc. 27:175-177(1994)

PubMed=32293552; DOI=10.1186/s40659-020-00282-7
Garcia P., Bizama C., Rosa L., Espinoza J.A., Weber H., Cerda-Infante J., Sanchez M., Montecinos V.P., Lorenzo-Bermejo J., Boekstegers F., Davila-Lopez M., Alfaro F., Leiva-Acevedo C., Parra Z., Romero D., Kato S., Leal P., Lagos M., Roa J.C.
Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor.
Biol. Res. 53:13.1-13.17(2020)

PubMed=32899426; DOI=10.3390/cancers12092510
Scherer D., Davila Lopez M., Goeppert B., Abrahamsson S., Gonzalez Silos R., Nova I., Marcelain K., Roa J.C., Ibberson D., Umu S.U., Rounge T.B., Roessler S., Bermejo J.L.
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling.
Cancers (Basel) 12:2510.1-2510.14(2020)

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