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HCC1937人乳腺癌细胞[STR鉴定正确]

产品型号:BH-C178
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C178

细胞英文(简称)

HCC1937

细胞名称

HCC1937人乳腺癌细胞

背景资料

这株细胞19951013日最初来源于原发性导管癌, 用了11.5个月建株。 肿瘤分类为TNM IIB期,3级。 BRCA1分析表明这株细胞是BRCA1 5382C 突变纯合的,而来源于同一病人的类淋巴母细胞细胞株在这个突变位点上是杂合的。另两个家庭成员也有这个突变;一个同卵双生姐妹也患有乳腺癌。这株细胞有一个后天的TP53突变, 而其野生型等位基因丢失;一个PTEN基因的后天的纯合缺失,以及多个与乳腺癌发病机理相关的位点上发生的杂合突变。这株细胞Her2-neup53表达都呈阴性。HCC1937的上皮细胞特异性标志上皮细胞糖蛋2(EGP2)和细胞角蛋白19都呈阳性。雌激素受体(ER)和孕酮(PR)表达阴性。

细胞来源

ATCC CRL-2336

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

乳房;原发性导管癌

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

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