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Kasumi-1人急性淋巴白血病细胞[STR鉴定正确]

产品型号:BH-C184
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C184

细胞英文(简称)

Kasumi-1

细胞名称

Kasumi-1;人急性淋巴白血病细胞【已通过STR鉴定】

背景资料

这是一个带有821号染色体转位的白血病细胞株。这个转位使得AML1基因和ETO (或称MTG8)基因串联,使融合基因AML1-ETO (也称作AML1-MTGRUNX1-CBF2T1)的表达升高,因而细胞产生嵌合的AML1-ETO蛋白。这个蛋白下调CEBPA mRNA,蛋白和DNA的结合活性,而这种结合对粒性白细胞的分化是极端重要的。这株细胞建立于一位急性白血病患者的外周血。这株细胞髓过氧化物酶阳性,显示其髓性成熟的形态。 增生试验显示,培养的细胞对IL-3IL-6G-CSFGM-CSF有反应,但IL-1IL-5没有反应。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

淋巴

细胞形态

悬浮

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +20% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

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