在线客服

晓洁:

198-8659-7060

HCE-T人角膜上皮细胞[STR鉴定正确]

产品型号:BH-C310
产品规格:1x10^6cell / T25瓶
产品价格:¥2450

返回列表

商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C310

细胞英文(简称)

HCE-T

细胞名称

HCE-T;人角膜上皮细胞【已通过STR鉴定】

背景资料

SV40腺病毒载体转化的角膜细胞。产生64kD的角蛋白。需用无霍乱毒素培养基。

细胞来源

RCB

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

角膜上皮

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM/F12+10% FBS+10ng/mL hEGF+5ug/mL Insu-lin+1% P/S

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=7534282
Araki-Sasaki K., Ohashi Y., Sasabe T., Hayashi K., Watanabe H., Tano Y., Handa H.
An SV40-immortalized human corneal epithelial cell line and its characterization.
Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26900324
Akin D., Newman J.R.B., McIntyre L.M., Sugrue S.P.
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.
Mol. Vis. 22:40-60(2016)

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018
Juretic M., Jurisic Dukovski B., Krtalic I., Reichl S., Cetina-Cizmek B., Filipovic-Grcic J., Lovric J., Pepic I.
HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?
Eur. J. Pharm. Sci. 104:23-30(2017)

PubMed=29787296; DOI=10.1080/02713683.2018.1480043
McDermott A.M., Baidouri H., Woodward A.M., Kam W.R., Liu Y., Chen X., Ziemanski J.F., Vistisen K., Hazlett L.D., Nichols K.K., Argueso P., Sullivan D.A.
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

联系我们
  • 19886597060