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L6 大鼠成肌细胞

产品型号:BH-C626
产品规格:冻存管/培养瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C626

细胞英文(简称)

L6

细胞名称

L6;大鼠成肌细胞  

背景资料

L6 肌原系最初是由 Yaffe 从在甲基胆蒽存在下维持前两代的大鼠大腿肌肉的原代培养物中分离出来的。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

骨胳肌

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS+1%P/S375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=5245982; DOI=10.1073/pnas.61.2.477
Yaffe D.
Retention of differentiation potentialities during prolonged cultivation of myogenic cells.
Proc. Natl. Acad. Sci. U.S.A. 61:477-483(1968)

PubMed=5481965; DOI=10.1016/S0012-1606(70)80004-5
Richler C., Yaffe D.
The in vitro cultivation and differentiation capacities of myogenic cell lines.
Dev. Biol. 23:1-22(1970)

PubMed=7297605; DOI=10.1016/0014-4827(81)90032-X
Parfett C.L.J., Jamieson J.C., Wright J.A.
A correlation between loss of fusion potential and defective formation of mannose-linked lipid intermediates in independent concanavalin A-resistant myoblast cell lines.
Exp. Cell Res. 136:1-14(1981)

PubMed=1311324; DOI=10.1016/S0021-9258(18)42923-7
Mitsumoto Y., Klip A.
Development regulation of the subcellular distribution and glycosylation of GLUT1 and GLUT4 glucose transporters during myogenesis of L6 muscle cells.
J. Biol. Chem. 267:4957-4962(1992)

PubMed=10484371; DOI=10.1152/ajpendo.1999.277.3.E572
Ueyama A., Yaworsky K.L., Wang Q., Ebina Y., Klip A.
GLUT-4myc ectopic expression in L6 myoblasts generates a GLUT-4-specific pool conferring insulin sensitivity.
Am. J. Physiol. 277:E572-E578(1999)

PubMed=33117511; DOI=10.4081/ejtm.2020.9306
Yablonka-Reuveni Z., Stockdale F.E., Nudel U., Israeli D., Blau H.M., Shainberg A., Neuman S., Kessler-Icekson G., Krull E.M., Paterson B., Fuchs O.S., Greenberg D., Sarig R., Halevy O., Ozawa E., Katcoff D.J.
Farewell to professor David Yaffe -- a pillar of the myogenesis field.
Eur. J. Transl. Myol. 30:9306.1-9306.16(2020)

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