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MDBK 牛肾细胞

产品型号:BH-C724
产品规格:冻存管/培养瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C723

细胞英文(简称)

MDBK

细胞名称

MDBK;牛肾细胞

背景资料

收到细胞,请查看瓶子是否有破裂,培养基是否漏出,是否浑浊,如有请尽快和我们联系。如包装完好,取出25cm2培养瓶,75%酒精消毒,拆下封口膜,在显微镜下观察细胞形态是否正常,细胞的贴壁情况以及细胞密度,然后放入375%CO2细胞培养箱中静置2-3小时,以稳定细胞状态。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

MEM +10% FBSC2056-1A+1%P/S375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=13567776; DOI=10.3181/00379727-98-24111
Madin S.H., Darby N.B. Jr.
Established kidney cell lines of normal adult bovine and ovine origin.
Proc. Soc. Exp. Biol. Med. 98:574-576(1958)

PubMed=14207851; DOI=10.1093/jnci/33.2.347
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Preservation of bulk chromatin with decrease in number of chromosomes in cells of an established bovine kidney line.
J. Natl. Cancer Inst. 33:347-361(1964)

PubMed=5988125; DOI=10.1007/BF00326444
Nelson-Rees W.A., Darby N.B. Jr., Siegel S.T.
Clonal derivatives of a bovine kidney cell line (MDBK) and continuing preservation of total chromosomal material.
Chromosoma 18:70-78(1966)

PubMed=5587551; DOI=10.1159/000129961
Nelson-Rees W.A., Kniazeff A.J., Darby N.B. Jr.
Debut and accumulation of centric fusion products: an index to age of certain cell lines.
Cytogenetics 6:436-450(1967)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=2580922; DOI=10.1089/jir.1985.5.65
Feinstein S., Traub A., Lazar A., Mizrahi A., Teitz Y.
Studies on cell binding and internalization of human lymphoblastoid (Namalva) interferon.
J. Interferon Res. 5:65-76(1985)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=24973239; DOI=10.1099/vir.0.065995-0
Richter M., Reimann I., Schirrmeier H., Kirkland P.D., Beer M.
The viral envelope is not sufficient to transfer the unique broad cell tropism of Bungowannah virus to a related pestivirus.
J. Gen. Virol. 95:2216-2222(2014)

PubMed=25565633; DOI=10.1371/journal.pone.0117154
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)

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