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CHO-S中国仓鼠卵巢细胞

产品型号:BH-C734
产品规格:1x10^6cell / T25瓶
产品价格:¥2800

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C734

细胞英文(简称)

CHO-S

细胞名称

CHO-S中国仓鼠卵巢细胞

背景资料

收到细胞,请查看瓶子是否有破裂,培养基是否漏出,是否浑浊,如有请尽快和我们联系。如包装完好,取出25cm2培养瓶,75%酒精消毒,拆下封口膜,在显微镜下观察细胞形态是否正常,细胞的贴壁情况以及细胞密度,然后放入375%CO2细胞培养箱中静置2-3小时,以稳定细胞状态。

细胞来源

Lonza

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

卵巢

细胞形态

悬浮

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

CHO悬浮细胞专用培养基375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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Clonal variation in colony morphology and growth of CHO cells cultured on agar.
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Wurm F.M.
CHO quasispecies -- implications for manufacturing processes.
Processes 1:296-311(2013)

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Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome.
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A signature of 12 microRNAs is robustly associated with growth rate in a variety of CHO cell lines.
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DOI=10.3390/pr5020020
Wurm F.M., Wurm M.J.
Cloning of CHO cells, productivity and genetic stability -- a discussion.
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PubMed=28876938; DOI=10.1021/acs.jproteome.7b00382
Heffner K.M., Hizal D.B., Yerganian G., Kumar A., Can O., O'Meally R., Cole R., Chaerkady R., Wu H., Bowen M.A., Betenbaugh M.J.
Lessons from the hamster: Cricetulus griseus tissue and CHO cell line proteome comparison.
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PubMed=32985598; DOI=10.1038/s41598-020-72959-8
Heffner K.M., Hizal D.B., Majewska N.I., Kumar S., Dhara V.G., Zhu J., Bowen M.A., Hatton D., Yerganian G., Yerganian A., O'Meally R., Cole R., Betenbaugh M.J.
Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities.
Sci. Rep. 10:15841-15841(2020)

PubMed=34050613; DOI=10.1002/biot.202100165
Wurm M.J., Wurm F.M.
Naming CHO cells for bio-manufacturing: genome plasticity and variant phenotypes of cell populations in bioreactors question the relevance of old names.
Biotechnol. J. 16:e2100165.1-e2100165.13(2021)

PubMed=36610518; DOI=10.1016/j.ymben.2022.12.009
Kotidis P., Donini R., Arnsdorf J., Hansen A.H., Voldborg B.G.R., Chiang A.W.T., Haslam S.M., Betenbaugh M.J., Jimenez Del Val I., Lewis N.E., Krambeck F., Kontoravdi C.
CHOGlycoNET: comprehensive glycosylation reaction network for CHO cells.
Metab. Eng. 76:87-96(2023)

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