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THLE-2人正常肝细胞

产品型号:BH-C2020
产品规格:1x10^6cell / T25瓶
产品价格:¥2800

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C2020

细胞英文(简称)

THLE-2

细胞名称

THLE-2;人正常肝细胞

背景资料

THLE-2 是一种从供体左叶分离的上皮细胞。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

2

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

专用培养基;375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

相关试剂

参考文献

DOI=10.1007/978-1-4612-0411-4_31
Lechner J.F., Smoot D.T., Pfeifer A.M.A., Cole K.H., Weston A., Groopman J.D., Shields P.G., Tokiwa T., Harris C.C.
A non-tumorigenic human liver epithelial cell culture model for chemical and biological carcinogenesis investigations.
(In) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.307-321; Humana Press; New York (1990)

PubMed=7685115; DOI=10.1073/pnas.90.11.5123
Pfeifer A.M.A., Cole K.E., Smoot D.T., Weston A., Groopman J.D., Shields P.G., Vignaud J.-M., Juillerat M., Lipsky M.M., Trump B.F., Lechner J.F., Harris C.C.
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.
Proc. Natl. Acad. Sci. U.S.A. 90:5123-5127(1993)

PubMed=8504475; DOI=10.1093/carcin/14.5.833
Lehman T.A., Modali R., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.
p53 mutations in human immortalized epithelial cell lines.
Carcinogenesis 14:833-839(1993)

Patent=US5529920
Cole K.H., Lechner J.F., Reddel R.R., Harris C.C., Pfeifer A.M.A.
Human liver epithelial cell line and culture media therefor.
Patent number US5529920, 25-Jun-1996

Patent=US5759765
Harris C.C., Cole K.H., Lechner J.F., Reddel R.R.
Human liver epithelial cell lines.
Patent number US5759765, 02-Jun-1998

PubMed=23887712; DOI=10.1038/ncomms3218
Nault J.-C., Mallet M., Pilati C., Calderaro J., Bioulac-Sage P., Laurent C., Laurent A., Cherqui D., Balabaud C., Zucman-Rossi J.
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat. Commun. 4:2218.1-2218.7(2013)

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