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MDCK(NBL-2)犬肾细胞

产品型号:BH-C701
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C701

细胞英文(简称)

MDCKNBL-2

细胞名称

MDCKNBL-2;马-达二氏犬肾细胞系

背景资料

1958年,MadinSHDarbyNB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类

细胞来源

ATCC CCL-34

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=13901412; DOI=10.1126/science.138.3536.42
Green I.J.
Serial propagation of influenza B (Lee) virus in a transmissible line of canine kidney cells.
Science 138:42-43(1962)

PubMed=5918973; DOI=10.3181/00379727-122-31293
Gaush C.R., Hard W.L., Smith T.F.
Characterization of an established line of canine kidney cells (MDCK).
Proc. Soc. Exp. Biol. Med. 122:931-935(1966)

PubMed=222773; DOI=10.1083/jcb.81.3.635
Rindler M.J., Chuman L.M., Shaffer L., Saier M.H. Jr.
Retention of differentiated properties in an established dog kidney epithelial cell line (MDCK).
J. Cell Biol. 81:635-648(1979)

PubMed=6480288
Schroyens W., Bruyneel R., Tchao R., Leighton J., Dragonetti C., Mareel M.
Comparison of invasiveness and non-invasiveness of two epithelial cell lines in vitro.
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PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8
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Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.
Virology 307:90-97(2003)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=21819694
Omeir R.L., Teferedegne B., Foseh G.S., Beren J.J., Snoy P.J., Brinster L.R., Cook J.L., Peden K., Lewis A.M. Jr.
Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells.
Comp. Med. 61:243-250(2011)

PubMed=21982418; DOI=10.1186/1471-2121-12-43
Dukes J.D., Whitley P., Chalmers A.D.
The MDCK variety pack: choosing the right strain.
BMC Cell Biol. 12:43.1-43.4(2011)

PubMed=24058646; DOI=10.1371/journal.pone.0075014
Lugovtsev V.Y., Melnyk D., Weir J.P.
Heterogeneity of the MDCK cell line and its applicability for influenza virus research.
PLoS ONE 8:E75014-E75014(2013)

PubMed=25903999; DOI=10.1002/biot.201400388
Genzel Y.
Designing cell lines for viral vaccine production: where do we stand?
Biotechnol. J. 10:728-740(2015)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

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