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PK-15猪肾细胞

产品型号:BH-C706
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C706

细胞英文(简称)

PK-15PK15

细胞名称

PK-15PK15;猪肾细胞系

背景资料

该细胞PCV阳性

细胞来源

ATCC CCL-33

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

MEM +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=5962858
Pirtle E.C.
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)

PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)

PubMed=4151202
Tischer I., Rasch R., Tochtermann G.
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)

PubMed=6160900
Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)

PubMed=3173126
Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)

PubMed=2686830
Dulac G.C., Afshar A.
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)

PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)

PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=26456528; DOI=10.1126/science.aad1191
Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)

PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y., Xia X.-L., Sun H.-C.
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682-113682(2019)

PubMed=32258794; DOI=10.2478/jvetres-2020-0020
Yin M., Hu D.-F., Li P., Kong L.-Y., Ning H.-M., Yue F., Jiang J.-Q., Wang X.-N.
Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus.
J. Vet. Res. 64:9-14(2020)

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