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Marc-145非洲绿猴胚胎肾细胞

产品型号:BH-C714
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C714

细胞英文(简称)

Marc-145

细胞名称

Marc-145;非洲绿猴胚胎肾细胞

背景资料

收到细胞请检查包装是否有破损,如有异常请尽快与我们联系!

细胞来源

ATCC

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

MEM +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

首次建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=8257302; DOI=10.1007/BF01313785
Kim H.S., Kwang J., Yoon I.J., Joo H.S., Frey M.L.
Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.
Arch. Virol. 133:477-483(1993)

Patent=US5690940
Joo H.S.
Low pathogencity PRRS live virus vaccines and methods of preparation thereof.
Patent number US5690940, 25-Nov-1997

PubMed=17081295; DOI=10.1186/1743-422X-3-90
Cafruny W.A., Duman R.G., Wong G.H.W., Said S., Ward-Demo P., Rowland R.R.R., Nelson E.A.
Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell-to-cell transfer in cultured MARC-145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug-targeting of cell permissiveness to virus infection.
Virol. J. 3:90.1-90.17(2006)

PubMed=23148668; DOI=10.1186/1743-422X-9-267
Provost C., Jia J.J., Music N., Levesque C., Lebel M.-E., del Castillo J.R.E., Jacques M., Gagnon C.A.
Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line.
Virol. J. 9:267.1-267.14(2012)

PubMed=28046048; DOI=10.1371/journal.pone.0169391
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)

PubMed=29682543; DOI=10.1155/2018/4315861
Wu X.-J., Qi J., Cong X.-Y., Chen L., Hu Y., Yoo D.-W., Wang G.-S., Tian F.-L., Li F., Sun W.-B., Chen Z., Guo L.-H., Wu J.-Q., Li J., Wang J.-B., Zhao X.-M., Du Y.-J.
Establishment and characterization of a high and stable porcine CD163-expressing MARC-145 cell line.
BioMed Res. Int. 2018:4315861-4315861(2018)

PubMed=33268540; DOI=10.1128/JCM.01757-20
Yim-Im W., Huang H.-Y., Park J., Wang C., Calzada G., Gauger P., Harmon K., Main R., Zhang J.-Q.
Comparison of ZMAC and MARC-145 cell lines for improving porcine reproductive and respiratory syndrome virus isolation from clinical samples.
J. Clin. Microbiol. 59:e01757.20.1-e01757.20.15(2021)

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