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UMNSAH/DF-1鸡胚胎成纤维细胞

产品型号:BH-C717
产品规格:1x10^6cell/T25瓶
产品价格:¥1620

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C717

细胞英文(简称)

UMNSAH/DF-1

细胞名称

UMNSAH/DF-1;鸡胚胎成纤维细胞

背景资料

收到细胞请检查包装是否有破损,如有异常请尽快与我们联系!

细胞来源

ATCC CRL-12203

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

2

组织来源

胚胎

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S395% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002


参考文献

Patent=US5672485
Foster D.N., Foster L.K.
Immortalized cell lines for virus growth.
Patent number US5672485, 30-Sep-1997

PubMed=9721238; DOI=10.1006/viro.1998.9290
Himly M., Foster D.N., Bottoli I., Iacovoni J.S., Vogt P.K.
The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses.
Virology 248:295-304(1998)

PubMed=9721239; DOI=10.1006/viro.1998.9291
Schaefer-Klein J., Givol I., Barsov E.V., Whitcomb J.M., VanBrocklin M., Foster D.N., Federspiel M.J., Hughes S.H.
The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors.
Virology 248:305-311(1998)

PubMed=16313905; DOI=10.1016/j.febslet.2005.10.066
Christman S.A., Kong B.-W., Landry M.M., Kim H., Foster D.N.
Modulation of p53 expression and its role in the conversion to a fully immortalized chicken embryo fibroblast line.
FEBS Lett. 579:6705-6715(2005)

CLPUB00401
Arifin M.A., Azmir M., Maizirwan M., Raha A.R., Sharifah S.H., Aini I.
Comparison of growth rate and viability of DF-1 cell line in different culture media.
(In) Abstracts of the 2nd International Conference on Science and Technology; pp.1-5; Universiti Teknologi MARA; Pulau Pinang (2008)

PubMed=18638503; DOI=10.1016/j.jviromet.2008.06.019
Lee C.-W., Jung K., Jadhao S.J., Suarez D.L.
Evaluation of chicken-origin (DF-1) and quail-origin (QT-6) fibroblast cell lines for replication of avian influenza viruses.
J. Virol. Methods 153:22-28(2008)

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)

PubMed=22111699; DOI=10.1186/1471-2164-12-571
Kong B.-W., Lee J.Y., Bottje W.G., Lassiter K., Lee J., Foster D.N.
Genome-wide differential gene expression in immortalized DF-1 chicken embryo fibroblast cell line.
BMC Genomics 12:571.1-571.19(2011)

PubMed=32005267; DOI=10.1186/s12985-020-1286-6
Pham P.H., Leacy A., Deng L., Nagy E., Susta L.
Isolation of Ontario aquatic bird bornavirus 1 and characterization of its replication in immortalized avian cell lines.
Virol. J. 17:16.1-16.12(2020)

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