在线客服

晓洁:

198-8659-7060

PC-12(未分化)大鼠肾上腺嗜铬细胞瘤细胞(未分化)

产品型号:BH-C603
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

返回列表

商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C603

细胞英文(简称)

PC-12

细胞名称

PC-12PC12大鼠肾上腺嗜铬细胞瘤细胞(未分化)

背景资料

该细胞系来自可移植的雄性大鼠的肾上腺嗜铬细胞瘤;表达神经生长因子(NGF)受体,NGF可诱导该细胞产生可逆性的神经表型。该细胞可产生儿茶酚胺、多巴胺和去甲肾上腺素,但不合成肾上腺素。

细胞来源

上海中科院

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

1

组织来源

肾上腺嗜铬细胞瘤

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640+15%HS+5%FBS+1% P/S 37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=1065897; DOI=10.1073/pnas.73.7.2424
Greene L.A., Tischler A.S.
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Proc. Natl. Acad. Sci. U.S.A. 73:2424-2428(1976)

PubMed=682602
Tischler A.S., Greene L.A.
Morphologic and cytochemical properties of a clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Lab. Invest. 39:77-89(1978)

PubMed=6500171; DOI=10.1016/0303-7207(84)90102-3
Bethea C.L., Kozak S.L.
Effect of extracellular matrix on PC 12 cell shape and dopamine processing.
Mol. Cell. Endocrinol. 37:319-329(1984)

PubMed=2493070; DOI=10.1111/j.1471-4159.1989.tb02538.x
Katoh-Semba R., Oohira A., Sano M., Watanabe K., Kitajima S., Kashiwamata S.
Glycosaminoglycan composition of PC12 pheochromocytoma cells: a comparison with PC12D cells, a new subline of PC12 cells.
J. Neurochem. 52:889-895(1989)

PubMed=2759161; DOI=10.1016/0277-5379(89)90388-X
Chelmicka-Schorr E., Checinski M.E., Arnason B.G.W.
PC12 pheochromocytoma and sympathetic nervous system derived trophic factors augment growth of neuroblastoma.
Eur. J. Cancer Clin. Oncol. 25:1057-1059(1989)

PubMed=2187480; DOI=10.1089/dna.1990.9.221
Muller S.R., Sullivan P.D., Clegg D.O., Feinstein S.C.
Efficient transfection and expression of heterologous genes in PC12 cells.
DNA Cell Biol. 9:221-229(1990)

PubMed=15541571; DOI=10.1016/j.mce.2003.02.001
Eaton M.J., Duplan H.
Useful cell lines derived from the adrenal medulla.
Mol. Cell. Endocrinol. 228:39-52(2004)

PubMed=16223334; DOI=10.1290/0411077.1
Dixon D.N., Loxley R.A., Barron A., Cleary S., Phillips J.K.
Comparative studies of PC12 and mouse pheochromocytoma-derived rodent cell lines as models for the study of neuroendocrine systems.
In Vitro Cell. Dev. Biol. Anim. 41:197-206(2005)

PubMed=18005394; DOI=10.1111/j.1748-1716.2007.01805.x
Westerink R.H.S., Ewing A.G.
The PC12 cell as model for neurosecretion.
Acta Physiol. 192:273-285(2008)

PubMed=22538498; DOI=10.1159/000338793
Grozinsky-Glasberg S., Shimon I., Rubinfeld H.
The role of cell lines in the study of neuroendocrine tumors.
Neuroendocrinology 96:173-187(2012)

联系我们
  • 19886597060