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NIH/3T3小鼠胚胎成纤维细胞

产品型号:BH-C401
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C401

细胞英文(简称)

NIH/3T3

细胞名称

NIH/3T3小鼠胚胎细胞

背景资料

NIH/3T3是从NIH Swiss小鼠胚胎培养物中建立的高度接触性抑制的连续传代细胞株。为了培育在形态学特征上更适合于进行转化分析的亚株,建立的NIH/3T3细胞株又进行了5轮以上亚克隆。该细胞株对肉瘤病毒的转化灶形成和白血病病毒的繁殖高度敏感,对DNA转化及转染研究十分有用。鼠痘病毒阴性。

细胞来源

ATCC CRL-1658

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

胚胎

细胞形态

贴壁;成纤维细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM(高糖) +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

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Two NIH3T3 cell lines of different origins circulating in the world.
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Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
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Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
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Circadian profiling of the transcriptome in NIH/3T3 fibroblasts: comparison with rhythmic gene expression in SCN2.2 cells and the rat SCN.
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DOI=10.3917/bhesv.191.0045
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Mutation carriers: led by Spaniards in U.S. laboratories in the early 80s.
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PubMed=23321776; DOI=10.1369/0022155413476868
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First molecular cytogenetic high resolution characterization of the NIH 3T3 cell line by murine multicolor banding.
J. Histochem. Cytochem. 61:306-312(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

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