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TM4小鼠睾丸Sertoli细胞

产品型号:BH-C413
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C413

细胞英文(简称)

TM4

细胞名称

TM4正常小鼠睾丸Sertoli细胞

背景资料

收到细胞请检查包装是否有破损,如有异常请尽快与我们联系!

细胞来源

ATCC CRL-1715

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

睾丸

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM/F12+5%HS+2.5%FBS+1% P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=6774781; DOI=10.1095/biolreprod23.1.243
Mather J.P.
Establishment and characterization of two distinct mouse testicular epithelial cell lines.
Biol. Reprod. 23:243-252(1980)

PubMed=7046561; DOI=10.1111/j.1749-6632.1982.tb23161.x
Mather J.P., Zhuang L.-Z., Perez-Infante V., Phillips D.M.
Culture of testicular cells in hormone-supplemented serum-free medium.
Ann. N. Y. Acad. Sci. 383:44-68(1982)

PubMed=6544874; DOI=10.1016/s0022-5320(84)80065-9
Mather J.P., Phillips D.M.
Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture.
J. Ultrastruct. Res. 87:263-274(1984)

PubMed=15541572; DOI=10.1016/j.mce.2003.05.001
Rahman N.A., Huhtaniemi I.T.
Testicular cell lines.
Mol. Cell. Endocrinol. 228:53-65(2004)

PubMed=26900862; DOI=10.1159/000444157
Schmid M., Guttenbach M., Steinlein C., Wanner G., Houben A.
Cytogenetic characterization of the TM4 mouse Sertoli cell line. II chromosome microdissection, FISH, scanning electron microscopy, and confocal laser scanning microscopy.
Cytogenet. Genome Res. 147:135-143(2015)

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