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RAW264.7小鼠单核巨噬细胞白血病细胞

产品型号:BH-C415
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C415

细胞英文(简称)

RAW 264.7

细胞名称

RAW264.7 RAW 264.7小鼠单核巨噬细胞白血病细胞

背景资料

此细胞株源自Abelson鼠科白血病病毒诱导的肿瘤。 sIg-, Ia-抗原、Thy-1.2表面抗原阴性。此细胞株不分泌可检测到的病毒颗粒,XC斑点形成试验阴性。 可以胞饮中性红并吞噬乳胶颗粒与酵母聚糖。 可以抗体依赖性地分解绵羊红血球与肿瘤靶细胞。 LPSPPD处理2天可诱导分解红血球但对肿瘤靶细胞无作用。

细胞来源

ATCC TIB-71

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

Abelson鼠科白血病病毒诱导的肿瘤;单核细胞;巨噬细胞

细胞形态

贴壁;不规则圆形

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM (高糖)+10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=212198; DOI=10.1016/0092-8674(78)90101-0
Raschke W.C., Baird S., Ralph P., Nakoinz I.
Functional macrophage cell lines transformed by Abelson leukemia virus.
Cell 15:261-267(1978)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Leenen P.J.M., Jansen A.M.A.C., van Ewijk W.
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=19057837; DOI=10.1007/s00774-008-0018-6
Vincent C., Kogawa M., Findlay D.M., Atkins G.J.
The generation of osteoclasts from RAW 264.7 precursors in defined, serum-free conditions.
J. Bone Miner. Metab. 27:114-119(2009)

PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
The phagosomal proteome in interferon-gamma-activated macrophages.
Immunity 30:143-154(2009)

PubMed=22735334; DOI=10.1038/msb.2012.21
Bordbar A., Mo M.L., Nakayasu E.S., Schrimpe-Rutledge A.C., Kim Y.-M., Metz T.O., Jones M.B., Frank B.C., Smith R.D., Peterson S.N., Hyduke D.R., Adkins J.N., Palsson B.O.
Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.
Mol. Syst. Biol. 8:558-558(2012)

DOI=10.5897/IJBMBR2013.0154
Iloki Assanga S.B., Gil-Salido A.A., Lewis Lujan L.M., Rosas-Durazo A., Acosta-Silva A.L., Rivera-Castaneda E.G., Rubio-Pino J.L.
Cell growth curves for different cell lines and their relationship with biological activities.
Int. J. Biotechnol. Mol. Biol. Res. 4:60-70(2013)

PubMed=23430347; DOI=10.1007/s10616-013-9545-7
Almeida J.L., Hill C.R., Cole K.D.
Mouse cell line authentication.
Cytotechnology 66:133-147(2014)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=25504905; DOI=10.1002/pmic.201400431
Guo M., Hartlova A., Dill B.D., Prescott A.R., Gierlinski M., Trost M.
High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages.
Proteomics 15:3169-3174(2015)

PubMed=29889899; DOI=10.1371/journal.pone.0198943
Taciak B., Bialasek M., Braniewska A., Sas Z., Sawicka P., Kiraga L., Rygiel T., Krol M.
Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages.
PLoS ONE 13:E0198943-E0198943(2018)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

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