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3T3-L1小鼠胚胎成纤维细胞

产品型号:BH-C416
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C416

细胞英文(简称)

3T3-L1

细胞名称

3T3-L1小鼠胚胎成纤维细胞

背景资料

3T3-L1是从3T3细胞(Swiss albino)中经克隆分离得到的连续传代的亚系。该细胞从快速分裂到汇合和接触性抑制状态经历了前脂肪细胞到脂肪样细胞的转变。可产生甘油三酯,高浓度血清可增强细胞内脂肪累积。

细胞来源

ATCC CL-173

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

胚胎

细胞形态

贴壁;成纤维细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM (高糖)+10% CS+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

DOI=10.1016/0092-8674(74)90126-3
Green H., Kehinde O.
Sublines of mouse 3T3 cells that accumulate lipid.
Cell 1:113-116(1974)

PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0
Green H., Meuth M.
An established pre-adipose cell line and its differentiation in culture.
Cell 3:127-133(1974)

PubMed=165899; DOI=10.1016/0092-8674(75)90087-2
Green H., Kehinde O.
An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.
Cell 5:19-27(1975)

Patent=US4003789
Green H.
Triglyceride-accumulating clonal cell line.
Patent number US4003789, 18-Jan-1977

PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6
Levine J.F., Stockdale F.E.
3T3-L1 adipocytes promote the growth of mammary epithelium.
Exp. Cell Res. 151:112-122(1984)

PubMed=28176856; DOI=10.1038/srep42104
Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.
Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.
Sci. Rep. 7:42104-42104(2017)

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