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BALB/3T3 clone A31小鼠胚胎成纤维细胞

产品型号:BH-C429
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C429

细胞英文(简称)

BALB/3T3 clone A31

细胞名称

BALB/3T3 clone A31小鼠胚胎成纤维细胞

背景资料

该细胞来自14-17天的Balb/c小鼠胚胎,对单纯疱疹和人脊髓灰质炎病毒1敏感。

细胞来源

ATCC CCL-163

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

胚胎;成纤维细胞

细胞形态

贴壁;成纤维细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM (高糖)+10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=4301006; DOI=10.1002/jcp.1040720208
Aaronson S.A., Todaro G.J.
Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40.
J. Cell. Physiol. 72:141-148(1968)

PubMed=4359949; DOI=10.1128/JVI.12.4.711-720.1973
Benveniste R.E., Todaro G.J., Scolnick E.M., Parks W.P.
Partial transcription of murine type C viral genomes in BALB c cell lines.
J. Virol. 12:711-720(1973)

PubMed=10848989; DOI=10.1046/j.1432-1327.2000.01404.x
Salvat C., Acquaviva C., Scheffner M., Robbins I., Piechaczyk M., Jariel-Encontre I.
Molecular characterization of the thermosensitive E1 ubiquitin-activating enzyme cell mutant A31N-ts20. Requirements upon different levels of E1 for the ubiquitination/degradation of the various protein substrates in vivo.
Eur. J. Biochem. 267:3712-3722(2000)

PubMed=22576795; DOI=10.3892/or.2012.1763
Ashinuma H., Takiguchi Y., Kitazono S., Kitazono-Saitoh M., Kitamura A., Chiba T., Tada Y., Kurosu K., Sakaida E., Sekine I., Tanabe N., Iwama A., Yokosuka O., Tatsumi K.
Antiproliferative action of metformin in human lung cancer cell lines.
Oncol. Rep. 28:8-14(2012)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

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