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C3H/10T1/2, Clone 8小鼠胚胎成纤维细胞

产品型号:BH-C431
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C431

细胞英文(简称)

C3H/10T1/2, Clone8

细胞名称

C3H/10T1/2, Clone8小鼠胚胎成纤维细胞

背景资料

该细胞系是由C. Reznikoff等(1972)从C3H系小鼠胚胎细胞分离。此细胞对过度长满的细胞有丝分裂抑制非常敏感,在同源小鼠中不产生肿瘤,无自然转化的背景,也不含明显内源性转化鼠类白血病或肉瘤病毒。

细胞来源

ATCC

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

胚胎

细胞形态

贴壁;成纤维细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

MEM +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=4357355
Reznikoff C.A., Brankow D.W., Heidelberger C.
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
Cancer Res. 33:3231-3238(1973)

PubMed=4796800
Reznikoff C.A., Bertram J.S., Brankow D.W., Heidelberger C.
Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division.
Cancer Res. 33:3239-3249(1973)

PubMed=3152871; DOI=10.1007/BF01128016
Christie N.T., Sen P., Costa M.
Chromosomal alterations in cell lines derived from mouse rhabdomyosarcomas induced by crystalline nickel sulfide.
Biol. Met. 1:43-50(1988)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

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