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RGC-5小鼠视网膜神经节细胞

产品型号:BH-C435
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C435

细胞英文(简称)

RGC-5

细胞名称

RGC-5小鼠视网膜神经节细胞

背景资料

RGC-5细胞是应用Ψ2E1A病毒转染出生后1d大鼠RGC获得的永生细胞株

细胞来源

ATCC

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

视网膜

细胞形态

贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

F12K+10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=11165366; DOI=10.1016/S0169-328X(00)00224-2
Krishnamoorthy R.R., Agarwal P., Prasanna G., Vopat K., Lambert W., Sheedlo H.J., Pang I.-H., Shade D.L., Wordinger R.J., Yorio T., Clark A.F., Agarwal N.
Characterization of a transformed rat retinal ganglion cell line.
Brain Res. Mol. Brain Res. 86:1-12(2001)

Patent=US7303915
Levin L.A.
In vitro-differentiated retinal ganglion cells and method for producing same.
Patent number US7303915, 04-Dec-2007

PubMed=19443730; DOI=10.1167/iovs.09-3484
Van Bergen N.J., Wood J.P., Chidlow G., Trounce I.A., Casson R.J., Ju W.K., Weinreb R.N., Crowston J.G.
Recharacterization of the RGC-5 retinal ganglion cell line.
Invest. Ophthalmol. Vis. Sci. 50:4267-4272(2009)

PubMed=23975727; DOI=10.1167/iovs.13-12085
Krishnamoorthy R.R., Clark A.F., Daudt D., Vishwanatha J.K., Yorio T.
A forensic path to RGC-5 cell line identification: lessons learned.
Invest. Ophthalmol. Vis. Sci. 54:5712-5719(2013)

PubMed=24021353; DOI=10.1016/j.exer.2013.08.002
Clark A.F., Tamm E.R., Al-Ubaidi M.R., Hollyfield J.G.
On the use of immortalized ocular cell lines in vision research: the unfortunate story of RGC-5.
Exp. Eye Res. 116:433-433(2013)

PubMed=24472671; DOI=10.1016/j.exer.2013.10.012
Al-Ubaidi M.R.
RGC-5: are they really 661W? The saga continues.
Exp. Eye Res. 119:115-115(2014)

PubMed=24664692; DOI=10.1007/978-1-4614-3209-8_19
Sippl C., Tamm E.R.
What is the nature of the RGC-5 cell line?
Adv. Exp. Med. Biol. 801:145-154(2014)

PubMed=29203899; DOI=10.1038/s41598-017-17241-0
Sayyad Z., Sirohi K., Radha V., Swarup G.
661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.
Sci. Rep. 7:16855-16855(2017)

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