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目录号	BH-C436
细胞英文(简称）	S-180（S180）
细胞名称	S-180（S180）小鼠腹水瘤细胞
背景资料	收到细胞请检查包装是否有破损，如有异常请尽快与我们联系!
细胞来源	ATCC
代次	P3
规格	冻存管/培养基
细胞数	1x10^6 cells
生物安全级别	1
组织来源	腹水瘤
细胞形态	悬浮+贴壁；圆形
细胞活力	95％（Viability by Trypan Blue Exclusion）
细胞检测	细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
培养条件	RPMI-1640 +10% FBS（货号：C2056-1A）+1%P/S；37℃，5% CO2
传代方法	建议1:2-1:3 两天换液一次
冻存条件	无血清细胞冻存液（货号：S002）

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参考文献

PubMed=13035452; DOI=10.1093/jnci/13.5.1299
Dunham L.J., Stewart H.L.
A survey of transplantable and transmissible animal tumors.
J. Natl. Cancer Inst. 13:1299-1377(1953)

PubMed=13823721
Foley G.E., Drolet B.P., McCarthy R.E., Goulet K.A., Dokos J.M., Filler D.A.
Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines.
Cancer Res. 20:930-939(1960)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

PubMed=14221803
Foley G.E., Drolet B.P.
Loss of neoplastic properties in vitro. I. Observations with S-180 cell lines.
Cancer Res. 24:1461-1467(1964)

PubMed=366289
Abercrombie M., Turner A.A.
Contact reactions influencing cell locomotion of a mouse sarcoma in culture.
Med. Biol. 56:299-303(1978)

PubMed=1546443; DOI=10.1016/0165-2427(92)90107-2
Alfaro G., Lomeli C., Ocadiz R., Ortega V., Barrera R., Ramirez M., Nava G.
Immunologic and genetic characterization of S180, a cell line of murine origin capable of growing in different inbred strains of mice.
Vet. Immunol. Immunopathol. 30:385-398(1992)

晓洁：

S-180小鼠腹水瘤细胞

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