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WEHI-3小鼠血液细胞

产品型号:BH-C447
产品规格:1x10^6cell/T25瓶
产品价格:¥1320

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商品详情

目录号

BH-C447

细胞英文(简称)

WEHI-3

细胞名称

WEHI-3;小鼠血液细胞

背景资料

该细胞表达补体C3受体(C3R),产生溶菌酶、粒细胞集落刺激活性(CSA)、IL-34ng/mlLPS可以抑制WEHI-3的生长,更高浓度可以阻断其生长;30~40μg/ml右旋葡聚糖也可抑制其生长;可以吞噬乳胶颗粒,但无毒性;可吞噬酵母聚糖和BCG,并阻断该细胞的生长;该细胞仅表现出微弱的抗体依赖的细胞介导的细胞毒反应。鼠痘病毒阴性。

细胞来源

ATCC

代次

P3

规格

冻存管/培养基

细胞数

1x10^6 cells

生物安全级别

1

组织来源

血液

细胞形态

悬浮+少量贴壁

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS(货号:C2056-1A+1%P/S37℃5% CO2

传代方法

首次建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=4543620; DOI=10.3181/00379727-144-37688
Rollinghoff M., Warner N.L.
Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells.
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McKean D.J., Infante A.J., Nilson A., Kimoto M., Fathman C.G., Walker E., Warner N.L.
Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.
J. Exp. Med. 154:1419-1431(1981)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
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Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

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