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AtT-20 小鼠垂体瘤细胞

产品型号:BH-C477
产品规格:1x10^6cell / T25瓶
产品价格:¥1320

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C477

细胞英文(简称)

AtT-20

细胞名称

AtT-20小鼠神经胶质瘤细胞

背景资料

AtT-20细胞生长时不贴壁,而结成活细胞团。能抗脊髓灰质炎病毒。检测发现肢骨发育畸形病毒(鼠痘)阴性。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

2

组织来源

垂体;瘤

细胞形态

悬浮聚团;淋巴母细胞

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

RPMI-1640 +10% FBS+1%P/S375% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

参考文献

PubMed=13874682; DOI=10.1073/pnas.48.7.1184
Buonassisi V., Sato G., Cohen A.I.
Hormone-producing cultures of adrenal and pituitary tumor origin.
Proc. Natl. Acad. Sci. U.S.A. 48:1184-1190(1962)

DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=6317196; DOI=10.1016/0092-8674(83)90187-3
Moore H.-P.H., Walker M.D., Lee F., Kelly R.B.
Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell Intracellular storage, proteolytic processing, and secretion on stimulation.
Cell 35:531-538(1983)

PubMed=2177466; DOI=10.1007/BF02624442
Vedder H.
Serum-free culture of AtT 20 pituitary cells: a system for neuroendocrine studies under defined conditions.
In Vitro Cell. Dev. Biol. 26:1068-1072(1990)

PubMed=8383976; DOI=10.1677/jme.0.0100025
Farrell W.E., Stewart M.F., Clark A.J.L., Crosby S.R., Davis J.R.E., White A.
Glucocorticoid inhibition of ACTH peptides: small cell lung cancer cell lines are more resistant than pituitary corticotroph adenoma cells.
J. Mol. Endocrinol. 10:25-32(1993)

PubMed=15541569; DOI=10.1016/j.mce.2004.07.018
Ooi G.T., Tawadros N., Escalona R.M.
Pituitary cell lines and their endocrine applications.
Mol. Cell. Endocrinol. 228:1-21(2004)

PubMed=21214938; DOI=10.1186/1471-2164-12-14
Atwood B.K., Lopez J., Wager-Miller J., Mackie K., Straiker A.
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14.1-14.14(2011)

PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

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