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NCI-H295R人肾上腺皮质腺癌细胞[STR鉴定正确]

产品型号:BH-C371
产品规格:1x10^6cell / T25瓶
产品价格:¥1800

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商品详情相关试剂说明书下载参考文献

商品详情

目录号

BH-C371

细胞英文(简称)

NCI-H295R

细胞名称

NCI-H295R; 人肾上腺皮质腺癌细胞 【已通过STR鉴定】

背景资料

该细胞系源于多能肾上腺皮质癌细胞系NCI-H295,后者由Gazdar AF等建立,从肾上腺皮质的肿瘤中分离而来。NCI-H295细胞经改变培养条件获得了NCI-295R细胞,群体倍增时间从原来的5天减为2天。NCI-295细胞悬浮生长,而NCI-H295R呈单层贴壁生长。NCI-H295R细胞保留了产生雄激素的能力,对血管紧张素和钾离子有反应。

细胞来源

ATCC

代次

P3

规格

冻存管/培养瓶

细胞数

1x10^6 cells

生物安全级别

2

组织来源

细胞形态

贴壁;上皮细胞样

细胞活力

95%(Viability by Trypan Blue Exclusion

细胞检测

细胞不含有HIV-1HBVHCV、支原体、细菌、酵母和真菌

培养条件

DMEM/F12 +10% FBS+1%ITS-G+1%P/S37℃5% CO2

传代方法

建议1:2-1:3 两天换液一次

冻存条件

无血清细胞冻存液(货号:S002

相关试剂

参考文献

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Human adrenal corticocarcinoma NCI-H295R cells produce more androgens than NCI-H295A cells and differ in 3beta-hydroxysteroid dehydrogenase type 2 and 17,20 lyase activities.
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